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avidin d conjugated hrp  (Vector Laboratories)


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    Structured Review

    Vector Laboratories avidin d conjugated hrp
    Avidin D Conjugated Hrp, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 291 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/avidin+d+conjugated+hrp/Avidin+D%2C+Horseradish+Peroxidase+(Av-HRP)%2C+Concentrate%2C+for+IHC/pm41855092-70-9-21
    Average 93 stars, based on 291 article reviews
    avidin d conjugated hrp - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Incubation:

    Article Title: CTLA4-Ig reduces proliferation and inflammatory gene expression in muscle fibroblasts, corresponding to less fibrosis and inflammation in mdx muscular dystrophy.
    Article Snippet: After a series of three, 10-minute washes in PBS, the tissues were 219 probed with species-specific, biotin-conjugated secondary antibodies (Vector Laboratories) diluted in 220 PBS to 1:200 for 30 minutes at room temperature (horse anti-goat IgG, Cat# BA-9500, 221 RRID:AB_2336123; rabbit anti-rat IgG, Cat# BA-4001, RRID:AB_10015300; horse anti-rabbit IgG, 222 Cat# BA-1100, RRID:AB_2336201; goat anti-hamster IgG, Cat# BA-9100, RRID:AB_2336137). .. 223 Washes were repeated and sections were incubated with avidin D-conjugated HRP (1:1000 in PBS, 224 30 minutes at room temperature, Vector Laboratories Cat# A-2004, RRID:AB_2336507). .. The 225 peroxidase substrate, AEC (3-amino-9-ethylcarbazole), was applied following the manufacturer’s 226 protocol (Vector Laboratories Cat# SK-4200, RRID:AB_2336076) and staining was visualized as a red 227 Downloaded from journals.physiology.org/journal/ajpcell (2401:4900:1CE3:2A95:9498:68CE:6ED0:8BFE) on March 21, 2026.

    Article Title: Klotho gene silencing promotes pathology in the mdx mouse model of Duchenne muscular dystrophy
    Article Snippet: The sections were washed with PBS and probed with biotin-conjugated secondary antibodies (Vector Laboratories, 1:200) for 1 h at room temperature. .. Sections were subsequently washed with PBS and then incubated for 30 min with avidin D-conjugated HRP (Vector, 1:1000). .. Staining was visualized with the peroxidase substrate, 3-amino-9-ethylcarbazole (Vector), yielding a red reaction product.

    Article Title: Targeting a therapeutic LIF transgene to muscle via the immune system ameliorates muscular dystrophy.
    Article Snippet: The sections were washed with phosphate buffered saline (PBS) and probed with biotin-conjugated secondary antibodies (1:200, 30 min at RT, Vector Laboratories). .. Sections were then washed with PBS and incubated with avidin D-conjugated HRP (1:1000, 30 min at RT, Vector). .. Staining was visualized with the peroxidase substrate, 3-amino-9ethylcarbazole (Vector).

    Article Title: Modulation of Klotho expression in injured muscle perturbs Wnt signalling and influences the rate of muscle growth
    Article Snippet: The sections were washed with PBS and probed with biotin-conjugated secondary antibodies (1:200; 30 minutes at RT, Vector Laboratories). .. Sections were then washed with PBS and incubated with avidin D-conjugated HRP (1:1000; 30 minutes at RT, Vector) and staining was visualized with AEC. .. Immunohistochemistry for Klotho was performed similarly on serial sections, except the sections were fixed in 4% paraformaldehyde for 10 minutes, no antigen retrieval was performed and the primary antibody (rat anti-mouse Klotho; R&D Systems #MAB1819, clone 236214; RRID:AB_2131926) was applied overnight at 4 o C. In other immunohistochemical assays to assess numbers of myogenic cells at later stages of myogenesis, sections were prepared identically to the Pax7 labeling protocol, except mouse anti-MyoD (1:50) (BD Pharmingen #554130; RRID:AB_395255) or mouse anti-myogenin (1:50) (BD Pharmingen #556358; RRID:AB_396383) were used as primary antibodies.

    Avidin-Biotin Assay:

    Article Title: CTLA4-Ig reduces proliferation and inflammatory gene expression in muscle fibroblasts, corresponding to less fibrosis and inflammation in mdx muscular dystrophy.
    Article Snippet: After a series of three, 10-minute washes in PBS, the tissues were 219 probed with species-specific, biotin-conjugated secondary antibodies (Vector Laboratories) diluted in 220 PBS to 1:200 for 30 minutes at room temperature (horse anti-goat IgG, Cat# BA-9500, 221 RRID:AB_2336123; rabbit anti-rat IgG, Cat# BA-4001, RRID:AB_10015300; horse anti-rabbit IgG, 222 Cat# BA-1100, RRID:AB_2336201; goat anti-hamster IgG, Cat# BA-9100, RRID:AB_2336137). .. 223 Washes were repeated and sections were incubated with avidin D-conjugated HRP (1:1000 in PBS, 224 30 minutes at room temperature, Vector Laboratories Cat# A-2004, RRID:AB_2336507). .. The 225 peroxidase substrate, AEC (3-amino-9-ethylcarbazole), was applied following the manufacturer’s 226 protocol (Vector Laboratories Cat# SK-4200, RRID:AB_2336076) and staining was visualized as a red 227 Downloaded from journals.physiology.org/journal/ajpcell (2401:4900:1CE3:2A95:9498:68CE:6ED0:8BFE) on March 21, 2026.

    Article Title: Klotho gene silencing promotes pathology in the mdx mouse model of Duchenne muscular dystrophy
    Article Snippet: The sections were washed with PBS and probed with biotin-conjugated secondary antibodies (Vector Laboratories, 1:200) for 1 h at room temperature. .. Sections were subsequently washed with PBS and then incubated for 30 min with avidin D-conjugated HRP (Vector, 1:1000). .. Staining was visualized with the peroxidase substrate, 3-amino-9-ethylcarbazole (Vector), yielding a red reaction product.

    Article Title: Targeting a therapeutic LIF transgene to muscle via the immune system ameliorates muscular dystrophy.
    Article Snippet: The sections were washed with phosphate buffered saline (PBS) and probed with biotin-conjugated secondary antibodies (1:200, 30 min at RT, Vector Laboratories). .. Sections were then washed with PBS and incubated with avidin D-conjugated HRP (1:1000, 30 min at RT, Vector). .. Staining was visualized with the peroxidase substrate, 3-amino-9ethylcarbazole (Vector).

    Article Title: Modulation of Klotho expression in injured muscle perturbs Wnt signalling and influences the rate of muscle growth
    Article Snippet: The sections were washed with PBS and probed with biotin-conjugated secondary antibodies (1:200; 30 minutes at RT, Vector Laboratories). .. Sections were then washed with PBS and incubated with avidin D-conjugated HRP (1:1000; 30 minutes at RT, Vector) and staining was visualized with AEC. .. Immunohistochemistry for Klotho was performed similarly on serial sections, except the sections were fixed in 4% paraformaldehyde for 10 minutes, no antigen retrieval was performed and the primary antibody (rat anti-mouse Klotho; R&D Systems #MAB1819, clone 236214; RRID:AB_2131926) was applied overnight at 4 o C. In other immunohistochemical assays to assess numbers of myogenic cells at later stages of myogenesis, sections were prepared identically to the Pax7 labeling protocol, except mouse anti-MyoD (1:50) (BD Pharmingen #554130; RRID:AB_395255) or mouse anti-myogenin (1:50) (BD Pharmingen #556358; RRID:AB_396383) were used as primary antibodies.

    Staining:

    Article Title: Modulation of Klotho expression in injured muscle perturbs Wnt signalling and influences the rate of muscle growth
    Article Snippet: The sections were washed with PBS and probed with biotin-conjugated secondary antibodies (1:200; 30 minutes at RT, Vector Laboratories). .. Sections were then washed with PBS and incubated with avidin D-conjugated HRP (1:1000; 30 minutes at RT, Vector) and staining was visualized with AEC. .. Immunohistochemistry for Klotho was performed similarly on serial sections, except the sections were fixed in 4% paraformaldehyde for 10 minutes, no antigen retrieval was performed and the primary antibody (rat anti-mouse Klotho; R&D Systems #MAB1819, clone 236214; RRID:AB_2131926) was applied overnight at 4 o C. In other immunohistochemical assays to assess numbers of myogenic cells at later stages of myogenesis, sections were prepared identically to the Pax7 labeling protocol, except mouse anti-MyoD (1:50) (BD Pharmingen #554130; RRID:AB_395255) or mouse anti-myogenin (1:50) (BD Pharmingen #556358; RRID:AB_396383) were used as primary antibodies.



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